PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

With the increasing demand for protein-rich foods and the challenges associated with traditional sources, including those used in animal farming, this study investigated the effects of a 10% supplementation of black soldier fly larvae meal (BSFLM) on the adaptive immunity of mice immunized with recombinant chimeras composed of the alpha (CPA), beta (CPB), and epsilon (ETX) toxins of Clostridium perfringens. The results revealed that the inclusion of BSFLM in the diet did not compromise feed palatability, feed conversion ratio (FCR, 27.2 < 30.4), or animal weight gain (< 24.8 g). The vaccines induced a humoral immune response, and immunoglobulin levels remained unaffected by supplementation. Conversely, BSFLM significantly stimulated the transcription of mRNA for both anti-inflammatory (IL-10) and pro-inflammatory (IL-12 and IFN-γ) cytokines in stimulated splenocytes. These findings suggest that BSFLM supplementation improves vaccine-induced immune responses, especially cell-mediated ones, indicating potential for more efficient and sustainable animal production methods.

• Black soldier fly larva insect meal recombinant vaccine immunomodulation

Abstract in Portuguese:

Com o aumento da demanda por alimentos ricos em proteínas e os desafios associados às fontes tradicionais, incluindo aquelas utilizadas na criação de animais, este estudo investigou os efeitos da suplementação de 10% de farinha de larvas da mosca soldado negra (BSFLM) sobre a imunidade de camundongos imunizados com quimeras recombinantes compostas pelas toxinas alfa (CPA), beta (CPB) e épsilon (ETX) de Clostridium perfringens. Os resultados revelaram que a inclusão de BSFLM na dieta não comprometeu a palatabilidade do alimento, a taxa de conversão alimentar (FCR; 27,2 < 30,4) ou o ganho de peso dos animais (< 24,8 g). As vacinas induziram uma resposta imunológica humoral, e os níveis de imunoglobulinas permaneceram inalterados pela suplementação. Por outro lado, a BSFLM estimulou significativamente a transcrição de mRNA para citocinas anti-inflamatórias (IL-10) e pró-inflamatórias (IL-12 e IFN-γ) em esplenócitos estimulados. Esses achados sugerem que a suplementação com BSFLM melhora as respostas imunológicas induzidas por vacinas, especialmente as mediadas por células, indicando potencial para métodos de produção animal mais eficientes e sustentáveis.

• Larva da mosca soldado negra farinha de inseto vacina recombinante imunomodulação

Abstract in English:

ABSTRACT.- Inoe A.P., Pereira F.C., Stopiglia A.J. & Da-Silva C.F. 2007. Pharmacological immunomodulation enhances peripheral nerve regeneration. Pesquisa Veterinária Brasileira 27(9):363-369. Departamento de Anatomia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Cidade Universitária, Av. Prof. Lineu Prestes 2415, São Paulo, SP 055508-900, Brazil. E-mail: ana.paula@unipar.br To assess the effect of N-Acetylmuramyl-L-Alanyl-D-Isoglutamine MDP topically administrated on the regenerating peripheral neurons, twelve male C57BL/6J adult mice were equally distributed into three groups. Four mice underwent unilateral sciatic nerve transection and polyethylene tubulization, with a 4mm gap between the proximal and distal nerve stumps and were implanted with collagen + PBS (COL). Other four animals underwent the same surgical procedure but received collagen + MDP (COL/MDP) inside the prosthesis. Four animals were not operated and served as control group (NOR). After 4 weeks, the regenerated nerve cables were processed for total myelinated axon counting and myelinated fiber diameter measurement. The L5 dorsal root ganglion (DRG) was also removed and sectioned for sensory neurons counting and measurement. The results revealed significant difference (p<0.05) in axonal counting among the groups NOR (4,355±32), COL (1,869±289) and COL/MDP (2,430±223). There was a significant reduction in the axonal diameter in the operated groups (COL=3.38mm±1.16 and COL/MDP=3.54mm±1.16) compared to NOR (6.19mm±2.45). No difference was found in the number of DRG neurons between the experimental groups (COL=564±51; COL/MDP=514±56), which presented fewer sensory neurons compared to NOR (1,097±142). Data obtained indicate that locally applied MDP stimulates peripheral nerve regeneration in mice.

• N-Acetylmuramyl-L-Alanyl-D-Isoglutamine MDP nerve regeneration drug effect sciatic nerve surgery

Abstract in Portuguese:

ABSTRACT.- Inoe A.P., Pereira F.C., Stopiglia A.J. & Da-Silva C.F. 2007. Pharmacological immunomodulation enhances peripheral nerve regeneration. Pesquisa Veterinária Brasileira 27(9):363-369. Departamento de Anatomia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Cidade Universitária, Av. Prof. Lineu Prestes 2415, São Paulo, SP 055508-900, Brazil. E-mail: ana.paula@unipar.br To assess the effect of N-Acetylmuramyl-L-Alanyl-D-Isoglutamine MDP topically administrated on the regenerating peripheral neurons, twelve male C57BL/6J adult mice were equally distributed into three groups. Four mice underwent unilateral sciatic nerve transection and polyethylene tubulization, with a 4mm gap between the proximal and distal nerve stumps and were implanted with collagen + PBS (COL). Other four animals underwent the same surgical procedure but received collagen + MDP (COL/MDP) inside the prosthesis. Four animals were not operated and served as control group (NOR). After 4 weeks, the regenerated nerve cables were processed for total myelinated axon counting and myelinated fiber diameter measurement. The L5 dorsal root ganglion (DRG) was also removed and sectioned for sensory neurons counting and measurement. The results revealed significant difference (p<0.05) in axonal counting among the groups NOR (4,355±32), COL (1,869±289) and COL/MDP (2,430±223). There was a significant reduction in the axonal diameter in the operated groups (COL=3.38mm±1.16 and COL/MDP=3.54mm±1.16) compared to NOR (6.19mm±2.45). No difference was found in the number of DRG neurons between the experimental groups (COL=564±51; COL/MDP=514±56), which presented fewer sensory neurons compared to NOR (1,097±142). Data obtained indicate that locally applied MDP stimulates peripheral nerve regeneration in mice.