PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Roza M.R., Silva L.A.F., Januário A.L., Barriviera M., Oliveira A.C.S. & Fioravanti M.C.S. 2009. [Cone beam computed tomography in veterinary dentistry: Description and standardization of the technique.] Tomografia computadorizada de feixe cônico na odontologia veterinária: descrição e padronização da técnica. Pesquisa Veterinária Brasileira 29(8):617-624. Departamento de Medicina Veterinária, Escola de Veterinária, Universidade Federal de Goiás, Campus II Samambaia, Cx. Postal 131, Goiânia, GO 74001-970, Brazil. E-mail: marcelloroza@gmail.com Eleven dogs and four cats with buccodental alterations, treated in the Centro Veterinário do Gama, in Brasilia, DF, Brazil, were submitted to cone beam computed tomography. The exams were carried out in a i-CAT tomograph, using for image acquisition six centimeters height, 40 seconds time, 0.2 voxel, 120 kilovolts and 46.72 milliamperes per second. The ideal positioning of the animal for the exam was also determined in this study and it proved to be fundamental for successful examination, which required a simple and safe anesthetic protocol due to the relatively short period of time necessary to obtain the images. Several alterations and diseases were identified with accurate imaging, demonstrating that cone beam computed tomography is a safe, accessible and feasible imaging method which could be included in the small animal dentistry routine diagnosis.

• Cone beam computed tomography

Abstract in Portuguese:

RESUMO.- Roza M.R., Silva L.A.F., Januário A.L., Barriviera M., Oliveira A.C.S. & Fioravanti M.C.S. 2009. [Cone beam computed tomography in veterinary dentistry: Description and standardization of the technique.] Tomografia computadorizada de feixe cônico na odontologia veterinária: descrição e padronização da técnica. Pesquisa Veterinária Brasileira 29(8):617-624. Departamento de Medicina Veterinária, Escola de Veterinária, Universidade Federal de Goiás, Campus II Samambaia, Cx. Postal 131, Goiânia, GO 74001-970, Brazil. E-mail: marcelloroza@gmail.com Onze cães e quatro gatos, portadores de alterações buco-dentárias e atendidos no Centro Veterinário do Gama, em Brasília, DF, foram submetidos à tomografia computadorizada de feixe cônico. Os exames foram realizados em um tomógrafo i-CAT, utilizando para aquisição das imagens, altura de seis centímetros, tempo de 40 segundos, 0,2 voxel, 120 kilovolts e 46,72 miliampéres por segundo. O melhor posicionamento dos animais para realização do exame foi definido neste estudo. Esse é um fator fundamental para a realização do exame, que necessitou um protocolo anestésico simples e seguro, em função do tempo mínimo necessário à obtenção das imagens. Várias alterações e enfermidades foram identificadas, com extrema acurácia, credenciando a tomografia computadorizada de feixe cônico como um exame seguro, acessível e exeqüível e que pode ser incorporado à rotina odontológica das clínicas de pequenos animais.

Abstract in English:

ABSTRACT.- Ferro D.G. & Gioso M.A. 2009. [Clinical study of effectiveness of an Anorganic Bone Matrix and Cell-Binding Peptide (ABM/P-15), PepGen-P15®: Application in advanced periodontal lesions of dogs.] Estudo clínico da aplicação de matriz inorgânica de osso associado a peptídeo sintético de adesão celular (MIO/P-15), PepGen P-15®, em lesões periodontais avançadas de cães. Pesquisa Veterinária Brasileira 29(2):109-116. Laboratório de Odontologia Comparada, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-900, Brazil. E-mail: deferro@usp.br The development of biologic modalities designed to enhance bone regeneration and wound healing of specific periodontal sites continues to be a challenge and has been accomplished through the cell binding activity of Type-I collagen. These have been provided by a synthetic cell biding peptide (P-15), associated to a anorganic bone matrix (ABM) to form ABM/P-15. The aim of this study was to evaluate the attachment loss and periodontal pocket response in teeth after 3 and 6 months with ABM/P-15 graft application. Twenty one dogs from the Veterinary Hospital, University of São Paulo, were anesthetized in order to accomplish periodontal treatment and 132 teeth faces with attachment loss were treated. From these, 36.4% (48 faces) received cell binding peptide and 63.6% (84 faces) compounded the control group that received conventional treatment (muco-gingival flap and root planning). The procedure was documented by intra-oral radiography and all periodontal probings were photographed. After 3 and 6 months, the animals were re-anesthetized in order to accomplish new photography, radiography and periodontal probing exams. The 48 attachment loss faces that received graft material exhibited 40% of regeneration rate after 6 months. The control faces did not change their attachment level. The palatal face presented the better regeneration rates (40%) and the canines and molars teeth showed the better responses (57.14% and 65%, respectively). There was no post-surgical infection related to absence of oral home care. It can be concluded that ABM/P-15 helps a more rapidly periodontal structure re-attachment and regeneration, including alveolar bone. Its application was easy and practical, and the post-surgical complications incidence was low. Nevertheless, more work is necessary to evaluate the amount and the quality of formed bone and periodontal ligament.

• Veterinary dentistry periodontal disease animal graft animal bone implant bone regeneration type I Collagen P-15 cell-binding peptide dog

Abstract in Portuguese:

ABSTRACT.- Ferro D.G. & Gioso M.A. 2009. [Clinical study of effectiveness of an Anorganic Bone Matrix and Cell-Binding Peptide (ABM/P-15), PepGen-P15®: Application in advanced periodontal lesions of dogs.] Estudo clínico da aplicação de matriz inorgânica de osso associado a peptídeo sintético de adesão celular (MIO/P-15), PepGen P-15®, em lesões periodontais avançadas de cães. Pesquisa Veterinária Brasileira 29(2):109-116. Laboratório de Odontologia Comparada, Departamento de Cirurgia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-900, Brazil. E-mail: deferro@usp.br The development of biologic modalities designed to enhance bone regeneration and wound healing of specific periodontal sites continues to be a challenge and has been accomplished through the cell binding activity of Type-I collagen. These have been provided by a synthetic cell biding peptide (P-15), associated to a anorganic bone matrix (ABM) to form ABM/P-15. The aim of this study was to evaluate the attachment loss and periodontal pocket response in teeth after 3 and 6 months with ABM/P-15 graft application. Twenty one dogs from the Veterinary Hospital, University of São Paulo, were anesthetized in order to accomplish periodontal treatment and 132 teeth faces with attachment loss were treated. From these, 36.4% (48 faces) received cell binding peptide and 63.6% (84 faces) compounded the control group that received conventional treatment (muco-gingival flap and root planning). The procedure was documented by intra-oral radiography and all periodontal probings were photographed. After 3 and 6 months, the animals were re-anesthetized in order to accomplish new photography, radiography and periodontal probing exams. The 48 attachment loss faces that received graft material exhibited 40% of regeneration rate after 6 months. The control faces did not change their attachment level. The palatal face presented the better regeneration rates (40%) and the canines and molars teeth showed the better responses (57.14% and 65%, respectively). There was no post-surgical infection related to absence of oral home care. It can be concluded that ABM/P-15 helps a more rapidly periodontal structure re-attachment and regeneration, including alveolar bone. Its application was easy and practical, and the post-surgical complications incidence was low. Nevertheless, more work is necessary to evaluate the amount and the quality of formed bone and periodontal ligament.