PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Alda J.L., Sallis E.S.V., Nogueira C.E.W., Soares M.P., Amaral L., Marcolongo-Pereira C., Frey Jr F. & Schild A.L. 2009. [Spontaneous Baccharis coridifolia (Compositae) poisoning in horses in southern Brazil.] Intoxicação espontânea por Baccharis coridifolia (Compositae) em eqüinos no Rio Grande do Sul. Pesquisa Veterinária Brasileira 29(5):409-414. Laboratório Regional de Diagnóstico, Faculdade de Veterinária, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS 96010-900, Brazil. E-mail: alschild@terra.com.br An outbreak of spontaneous Baccharis coridifolia poisoning in horses in July 2008 is reported from southern Brazil. The poisoning affected three mares out of four that were transported from the state of Paraná to a farm in the municipality of Acegua, Rio Grande do Sul, and occurred 3 days after arrival of the animals in a paddock with sprouting B. coridifolia. The fourth mare introduced to another paddock without B. coridifolia was not affected. The mares had received only one third of the concentrate ration previously ingested and stayed during the night in a stall. Clinical signs were abdominal discomfort, increased cardiac and respiratory rate, anorexia, hypermotility of the gut, cecal tympany, and diarrhea. The clinical course was of 18-36 hours. One affected mare survived after symptomatic treatment. Gross lesions were severe congestion, hemorrhages, edema and ulcers of the glandular stomach. Congestion, edema and hemorrhages were also observed in ileum, cecum and large colon. Histologic examination revealed degeneration and necrosis of the aglandular epithelium of the stomach, gastritis and enteritis with infiltration by mononuclear cells and neutrophils, edema of the mucosa, and dilatation of lymphatic vessels. One horse was poisoned experimentally with 1g/kg body weight of B. coridifolia. Clinical signs and lesions were similar to those observed in the spontaneous cases.

• Baccharis coridifolia poisonous plants diseases of horses plant poisoning Compositae Plantas tóxicas intoxicação por plantas

Abstract in Portuguese:

ABSTRACT.- Alda J.L., Sallis E.S.V., Nogueira C.E.W., Soares M.P., Amaral L., Marcolongo-Pereira C., Frey Jr F. & Schild A.L. 2009. [Spontaneous Baccharis coridifolia (Compositae) poisoning in horses in southern Brazil.] Intoxicação espontânea por Baccharis coridifolia (Compositae) em eqüinos no Rio Grande do Sul. Pesquisa Veterinária Brasileira 29(5):409-414. Laboratório Regional de Diagnóstico, Faculdade de Veterinária, Universidade Federal de Pelotas, Campus Universitário s/n, Pelotas, RS 96010-900, Brazil. E-mail: alschild@terra.com.br An outbreak of spontaneous Baccharis coridifolia poisoning in horses in July 2008 is reported from southern Brazil. The poisoning affected three mares out of four that were transported from the state of Paraná to a farm in the municipality of Acegua, Rio Grande do Sul, and occurred 3 days after arrival of the animals in a paddock with sprouting B. coridifolia. The fourth mare introduced to another paddock without B. coridifolia was not affected. The mares had received only one third of the concentrate ration previously ingested and stayed during the night in a stall. Clinical signs were abdominal discomfort, increased cardiac and respiratory rate, anorexia, hypermotility of the gut, cecal tympany, and diarrhea. The clinical course was of 18-36 hours. One affected mare survived after symptomatic treatment. Gross lesions were severe congestion, hemorrhages, edema and ulcers of the glandular stomach. Congestion, edema and hemorrhages were also observed in ileum, cecum and large colon. Histologic examination revealed degeneration and necrosis of the aglandular epithelium of the stomach, gastritis and enteritis with infiltration by mononuclear cells and neutrophils, edema of the mucosa, and dilatation of lymphatic vessels. One horse was poisoned experimentally with 1g/kg body weight of B. coridifolia. Clinical signs and lesions were similar to those observed in the spontaneous cases.

Abstract in English:

Abstract.- Pedroso P.M.O., Bandarra P.M., Bezerra Júnior P.S., Raymundo D.L., Borba M.R., Leal J.S. & Driemeier D. 2009. [Natural and experimental poisoning by Nerium oleander (Apocynaceae) in cattle in Rio Grande do Sul.] Intoxicação natural e experimental por Nerium oleander (Apocynaceae) em bovinos no Rio Grande do Sul. Pesquisa Veterinária Brasileira 29(5):404-408. Setor de Patologia Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brazil. E-mail: davetpat@ufrgs.br This paper describes natural and experimental poisoning of cattle by Nerium oleander in Rio Grande do Sul. Two out of eight cattle died acutely after consumption of leaves of Nerium oleander, branches of which had been cut and placed into a paddock where the animals were kept. An affected cow did not show clinical signs, but a 4-month-old calf presented lateral recumbence, paddling, vocalization and death. Main gross findings in the cow naturally poisoned and in two experimentally intoxicated heifers were observed in the heart and included hemorrhages in the left atrium, clots and hemorrhages in the left ventricular endocardium, and pale areas in the interventricular septum and ventricular myocardium. Histologically, there was coagulation necrosis of individual cardiac fibers or small groups of fibers, characterized by enhanced cytoplasmic eosinophily and picnotic nuclei. These lesions were most severe in the papillary muscle. The diagnosis was based on presence of the trimmed N. oleander in the paddock where the animals stayed, evidence of consumption of the plant, consistent clinical and pathological findings, and experimental reproduction of the disease through oral administration of 0.5 and 1.0g/kg of its green leaves to two cattle.

• Nerium oleander plant poisoning poisonous plant intoxicação por planta plantas tóxicas Apocynaceae

Abstract in Portuguese:

Abstract.- Pedroso P.M.O., Bandarra P.M., Bezerra Júnior P.S., Raymundo D.L., Borba M.R., Leal J.S. & Driemeier D. 2009. [Natural and experimental poisoning by Nerium oleander (Apocynaceae) in cattle in Rio Grande do Sul.] Intoxicação natural e experimental por Nerium oleander (Apocynaceae) em bovinos no Rio Grande do Sul. Pesquisa Veterinária Brasileira 29(5):404-408. Setor de Patologia Veterinária, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Porto Alegre, RS 91540-000, Brazil. E-mail: davetpat@ufrgs.br This paper describes natural and experimental poisoning of cattle by Nerium oleander in Rio Grande do Sul. Two out of eight cattle died acutely after consumption of leaves of Nerium oleander, branches of which had been cut and placed into a paddock where the animals were kept. An affected cow did not show clinical signs, but a 4-month-old calf presented lateral recumbence, paddling, vocalization and death. Main gross findings in the cow naturally poisoned and in two experimentally intoxicated heifers were observed in the heart and included hemorrhages in the left atrium, clots and hemorrhages in the left ventricular endocardium, and pale areas in the interventricular septum and ventricular myocardium. Histologically, there was coagulation necrosis of individual cardiac fibers or small groups of fibers, characterized by enhanced cytoplasmic eosinophily and picnotic nuclei. These lesions were most severe in the papillary muscle. The diagnosis was based on presence of the trimmed N. oleander in the paddock where the animals stayed, evidence of consumption of the plant, consistent clinical and pathological findings, and experimental reproduction of the disease through oral administration of 0.5 and 1.0g/kg of its green leaves to two cattle.

Abstract in English:

ABSTRACT.- Barbosa J.D., Junior J.M.A., Pinheiro C.P., Oliveira C.H.S., Oliveira C.M.C., Sousa M.G.S. & Duarte M.D. 2009. [Acumulation of the seeds of Acrocomia aculeata (Arecaceae) in the cheeks of buffaloes in the State of Pará, Brazil.] Acúmulo de sementes de Acrocomia aculeata (Arecaceae) na bochecha de búfalos no Pará. Pesquisa Veterinária Brasileira 29(5):431-434. Central de Diagnóstico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal do Pará, Campus Castanhal, Rua Maximino Porpino da Silva 1000, Castanhal, PA 68740-080, Brazil. E-mail: diomedes@ufpa.br A condition of buffaloes characterized by an increased volume of one of the cheeks was shown to be due to accumulation of the seeds of the palm-tree “mucaja” (Acrocomia aculeata) and of grass, in the oral vestibulum during rumination. This caused compressive atrophy with thinning and medial deviation of the corresponding submaxillary bone and exposition of the roots of molar teeth. The seeds appear to be of good palatability to the buffaloes.

• Acrocomia aculeata Arecaceae diseases of buffaloes doenças de búfalos

Abstract in Portuguese:

ABSTRACT.- Barbosa J.D., Junior J.M.A., Pinheiro C.P., Oliveira C.H.S., Oliveira C.M.C., Sousa M.G.S. & Duarte M.D. 2009. [Acumulation of the seeds of Acrocomia aculeata (Arecaceae) in the cheeks of buffaloes in the State of Pará, Brazil.] Acúmulo de sementes de Acrocomia aculeata (Arecaceae) na bochecha de búfalos no Pará. Pesquisa Veterinária Brasileira 29(5):431-434. Central de Diagnóstico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal do Pará, Campus Castanhal, Rua Maximino Porpino da Silva 1000, Castanhal, PA 68740-080, Brazil. E-mail: diomedes@ufpa.br A condition of buffaloes characterized by an increased volume of one of the cheeks was shown to be due to accumulation of the seeds of the palm-tree “mucaja” (Acrocomia aculeata) and of grass, in the oral vestibulum during rumination. This caused compressive atrophy with thinning and medial deviation of the corresponding submaxillary bone and exposition of the roots of molar teeth. The seeds appear to be of good palatability to the buffaloes.

Abstract in English:

ABSTRACT.- Barbosa J.D., Silveira J.A.S, Albernaz T.T., Silva N.S., Belo Reis A.S., Oliveira C.M.C., Riet- Correa G. & Duarte M.D. 2009 [Cutaneous lesions of the legs caused by the thorns of Mimosa pudica (Leg. Mimosoideae) in cattle and sheep in the State of Pará.] Lesões de pele causadas por Mimosa pudica (Leg. Mimosoideae) nos membros de bovinos e ovinos no Estado do Pará. Pesquisa Veterinária Brasileira 29(5):435-438. Central de Diagnóstico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal do Pará, Campus Castanhal, Rua Maximino Porpino da Silva 1000, Castanhal, PA 68740-080, Brazil. E-mail: diomedes@ufpa.br A condition of cattle and sheep characterized by ulcerous and granulomatous skin lesions of the legs was studied. Epidemiological data and the nature of the lesions indicate that they are caused by the thorns of Mimosa pudica (Leg. Mimosoideae). The disease was observed only in pastures largely infested by the plant, and animals recover quickly when moved from them.

• Mimosa pudica Leguminosae Mimosoideae traumatic lesions malícia lesões traumáticas

Abstract in Portuguese:

ABSTRACT.- Barbosa J.D., Silveira J.A.S, Albernaz T.T., Silva N.S., Belo Reis A.S., Oliveira C.M.C., Riet- Correa G. & Duarte M.D. 2009 [Cutaneous lesions of the legs caused by the thorns of Mimosa pudica (Leg. Mimosoideae) in cattle and sheep in the State of Pará.] Lesões de pele causadas por Mimosa pudica (Leg. Mimosoideae) nos membros de bovinos e ovinos no Estado do Pará. Pesquisa Veterinária Brasileira 29(5):435-438. Central de Diagnóstico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal do Pará, Campus Castanhal, Rua Maximino Porpino da Silva 1000, Castanhal, PA 68740-080, Brazil. E-mail: diomedes@ufpa.br A condition of cattle and sheep characterized by ulcerous and granulomatous skin lesions of the legs was studied. Epidemiological data and the nature of the lesions indicate that they are caused by the thorns of Mimosa pudica (Leg. Mimosoideae). The disease was observed only in pastures largely infested by the plant, and animals recover quickly when moved from them.

Abstract in English:

ABSTRACT.- Barbosa J.D., Albernaz T.T., Riet-Correa G, Cerqueira V.D., Soares S.O., Campos K.F., Oliveira C.M.C. & Duarte M.D. 2009. [Angular cheilitis in horses associated with ingestion of Panicum maximum.] Queilite angular traumática em eqüinos associada à ingestão de Panicum maximum. Pesquisa Veterinária Brasileira 29(5):428-430. Central de Diagnóstico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal do Pará, Campus Castanhal, Rua Maximino Porpino da Silva 1000, Castanhal, PA 68740-080, Brazil. E-mail: diomedes@ufpa.br A condition with enlargement of the oral cleft in horses was studied. The enlargement of varied extension was uni or bilateral. The cheek mucosa of the labial commissure showed slight erosions. During chewing there was loss of small amounts of grass and saliva through the oral cleft. The affected horses were in good nutritional condition. Histopathological studies of tissues obtained by biopsia, revealed a superficial epidermitis. The pastures consisted of Panicum maximum grass (varieties Tanzânia, Mombaça, Tobiatã and Colonião) which was mature, tall, lignified, with leaves of cutting edges. Based on epidemiological, clinical and histopathological data, it was concluded that the lesions were caused by the hard grass, favored by the way horses pull the tall grass and chew it.

• Cheilitis Panicum maximum diseases of horses

Abstract in Portuguese:

ABSTRACT.- Barbosa J.D., Albernaz T.T., Riet-Correa G, Cerqueira V.D., Soares S.O., Campos K.F., Oliveira C.M.C. & Duarte M.D. 2009. [Angular cheilitis in horses associated with ingestion of Panicum maximum.] Queilite angular traumática em eqüinos associada à ingestão de Panicum maximum. Pesquisa Veterinária Brasileira 29(5):428-430. Central de Diagnóstico Veterinário, Faculdade de Medicina Veterinária, Universidade Federal do Pará, Campus Castanhal, Rua Maximino Porpino da Silva 1000, Castanhal, PA 68740-080, Brazil. E-mail: diomedes@ufpa.br A condition with enlargement of the oral cleft in horses was studied. The enlargement of varied extension was uni or bilateral. The cheek mucosa of the labial commissure showed slight erosions. During chewing there was loss of small amounts of grass and saliva through the oral cleft. The affected horses were in good nutritional condition. Histopathological studies of tissues obtained by biopsia, revealed a superficial epidermitis. The pastures consisted of Panicum maximum grass (varieties Tanzânia, Mombaça, Tobiatã and Colonião) which was mature, tall, lignified, with leaves of cutting edges. Based on epidemiological, clinical and histopathological data, it was concluded that the lesions were caused by the hard grass, favored by the way horses pull the tall grass and chew it.

Abstract in English:

ABSTRACT.- Silva K.P.C., Mota R.A., Cunha A.P., Silva L.B.G., Leal N.C., Cavalcante Y.V.N., Teles J.A.A., Pereira M.C.C. & Freitas N.S. 2009. [Phenotypic and molecular characterization of Burkholderia mallei isolated in northeastern Brazil.] Caracterização fenotípica e molecular de amostras de Burkholderia mallei isoladas na Região Nordeste do Brasil. Pesquisa Veterinária Brasileira 29(5):439-444. Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Dois Irmãos, Recife, PE 52171-900, Brazil. E-mail: rinaldo.mota@hotmail.com The objective of this paper was to study the molecular performance and phenotypic characterization of Burkholderia mallei isolated from horses with clinical and serological diagnosis of glanders, originating from the Metropolitan District of Recife and Zona da Mata of Pernambuco and Alagoas. The isolation and biochemical identification of B. mallei was carried out by microbiological and molecular techniques of PCR-fingerprinting and RAPD-PCR. From the eight samples studied, four showed little phenotype variations. In the molecular tests, the samples formed 4 groups of different ribotype profiles and 4 genotype profiles. There was some association of PCR-fingerprinting with RAPD-PCR results. It was concluded that the slight biochemical variations were not associated with different molecular profiles. They also indicated that these differences show heterogeneity associated with the origin of the sample, indicating that the infection was caused by clones of different strains and that the polymorphism of DNA observed could make it

• Burkholderia mallei glanders mormo doenças de eqüdeos

Abstract in Portuguese:

ABSTRACT.- Silva K.P.C., Mota R.A., Cunha A.P., Silva L.B.G., Leal N.C., Cavalcante Y.V.N., Teles J.A.A., Pereira M.C.C. & Freitas N.S. 2009. [Phenotypic and molecular characterization of Burkholderia mallei isolated in northeastern Brazil.] Caracterização fenotípica e molecular de amostras de Burkholderia mallei isoladas na Região Nordeste do Brasil. Pesquisa Veterinária Brasileira 29(5):439-444. Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Dois Irmãos, Recife, PE 52171-900, Brazil. E-mail: rinaldo.mota@hotmail.com The objective of this paper was to study the molecular performance and phenotypic characterization of Burkholderia mallei isolated from horses with clinical and serological diagnosis of glanders, originating from the Metropolitan District of Recife and Zona da Mata of Pernambuco and Alagoas. The isolation and biochemical identification of B. mallei was carried out by microbiological and molecular techniques of PCR-fingerprinting and RAPD-PCR. From the eight samples studied, four showed little phenotype variations. In the molecular tests, the samples formed 4 groups of different ribotype profiles and 4 genotype profiles. There was some association of PCR-fingerprinting with RAPD-PCR results. It was concluded that the slight biochemical variations were not associated with different molecular profiles. They also indicated that these differences show heterogeneity associated with the origin of the sample, indicating that the infection was caused by clones of different strains and that the polymorphism of DNA observed could make it

Abstract in English:

ABSTRACT.- Beserra P.S., Sales G.A., Santana E.J.M., Miranda S.A., Brito A.B., Nickolak E., Freitas V.V. & Domingues S.F.S. 2009. [B-Mode Ultrasound biometry of the ocular globe related with fronto-occiptal and bizigomatic diameters in Canis familiaris.] Relação entre a biometria ultra-sonográfica em modo B do globo ocular e os diâmetros fronto occiptal e bizigomático em Canis familiaris. Pesquisa Veterinária Brasileira 29(4):286-290. Laboratório de Biologia e Medicina de Animais da Amazônia, Medicina Veterinária, Universidade Federal do Pará, Rua Hernane Lameira n° 556, Castanhal, PA 68743-050, Brazil. E-mail: shfarha@ufpa.br In the experiment 31 healthy mongrel dogs, 8 months to 7 years of age, were used, 10 males and 21 females, weighing 1.5-28 kg. Initially, fronto-occipetal (FOD) and bizigomatic (BZD) diameters were measured using a caliper. The ophthalmologic transpalpebral B-mode ultrasonography (US) was performed to measure the internal ocular structures, as follows: The cornea thickness (D1), distance between cornea and anterior lens capsule (D2), distance between cornea and posterior lens capsule (D3), lens thickness (D4), lens diameter (D5), lens area (D6), distance between posterior lens capsule and retina (D7), distance between anterior lens capsule and retina (D8), and the distance between cornea and retina (D9). Except for D4, there were effect of FOD and BZD on the measures of the internal structures of GO. The Lineal Regression Analysis between the measures of the internal oculars structures and DFO and DBZ were significant for D1, D2, D3, D4, D5, D6, D7, D8 and D9.

• B-mode ultrasonography ocular biometry bizigomatic diameter fronto-occiptal diameter dogs

Abstract in Portuguese:

ABSTRACT.- Beserra P.S., Sales G.A., Santana E.J.M., Miranda S.A., Brito A.B., Nickolak E., Freitas V.V. & Domingues S.F.S. 2009. [B-Mode Ultrasound biometry of the ocular globe related with fronto-occiptal and bizigomatic diameters in Canis familiaris.] Relação entre a biometria ultra-sonográfica em modo B do globo ocular e os diâmetros fronto occiptal e bizigomático em Canis familiaris. Pesquisa Veterinária Brasileira 29(4):286-290. Laboratório de Biologia e Medicina de Animais da Amazônia, Medicina Veterinária, Universidade Federal do Pará, Rua Hernane Lameira n° 556, Castanhal, PA 68743-050, Brazil. E-mail: shfarha@ufpa.br In the experiment 31 healthy mongrel dogs, 8 months to 7 years of age, were used, 10 males and 21 females, weighing 1.5-28 kg. Initially, fronto-occipetal (FOD) and bizigomatic (BZD) diameters were measured using a caliper. The ophthalmologic transpalpebral B-mode ultrasonography (US) was performed to measure the internal ocular structures, as follows: The cornea thickness (D1), distance between cornea and anterior lens capsule (D2), distance between cornea and posterior lens capsule (D3), lens thickness (D4), lens diameter (D5), lens area (D6), distance between posterior lens capsule and retina (D7), distance between anterior lens capsule and retina (D8), and the distance between cornea and retina (D9). Except for D4, there were effect of FOD and BZD on the measures of the internal structures of GO. The Lineal Regression Analysis between the measures of the internal oculars structures and DFO and DBZ were significant for D1, D2, D3, D4, D5, D6, D7, D8 and D9.

Abstract in English:

BSTRACT.- Reis A.B., Sant’Ana D.M.G., Azevedo J.F., Merlini L.S. & Araújo E.J.A. 2009. [The influence of the aquatic environment in tanks sequetially interconnected with PVC pipes on the gill epithelium and lamellas of tilapia (Oreochromis niloticus).] Alterações do epitélio branquial e das lamelas de tilápias (Oreochromis niloticus) causadas por mudanças do ambiente aquático em tanques de cultivo intensivo. Pesquisa Veterinária Brasileira 29(4):303-311. Laboratório de Neurogastroenterologia Experimental, Universidade Paranaense, Praça Mascarenhas de Moraes 4286, Umuarama, PR 87502-210, Brazil. E-mail: eduardoaraujo@unipar.br The behavior of the gill epithelium of tilapias cultured in tanks at different altitudes and interconnected with PVC pipes was analyzed. Gill filaments of four specimens from four tanks (T1, T2, T3 e T4) sequentially interconnected were submitted to histological routine to obtain 5-mm-thick cuts that were stained with HE or submitted to histochemistry reactions PAS + diastase solution or Alcian Blue pH 2.5 or Alcian Blue pH 1.0. Considering the intermediary, apical and basal regions of the filaments, the lamellar area was measured and the amount of mucous cells was counted. It was verified that oxygen, pH, and temperature decreased progressively as the water flew from one tank to another. Thus, an increase was realized of the amount of mucous cells and the lamellar area in T2, as well as a progressive decrease of these measures on the tanks which received water from T2. Moreover, detachment of the gill epithelium, cellular hyperplasia in the interlamellar space, and telangectasias were observed in the fishes from T2, T3 e T4. It was concluded that the aquatic environment in tanks sequentially interconnected with PVC pipes suffers alterations from one tank to another, as that physico-chemical fluctuations reflect on the behavior of the gill epithelium through variations of the lamellar area and the amount of mucous cells.

• Nile tilapia intensive culture system gill; morphometry mucous cells secundary lamellar cells

Abstract in Portuguese:

BSTRACT.- Reis A.B., Sant’Ana D.M.G., Azevedo J.F., Merlini L.S. & Araújo E.J.A. 2009. [The influence of the aquatic environment in tanks sequetially interconnected with PVC pipes on the gill epithelium and lamellas of tilapia (Oreochromis niloticus).] Alterações do epitélio branquial e das lamelas de tilápias (Oreochromis niloticus) causadas por mudanças do ambiente aquático em tanques de cultivo intensivo. Pesquisa Veterinária Brasileira 29(4):303-311. Laboratório de Neurogastroenterologia Experimental, Universidade Paranaense, Praça Mascarenhas de Moraes 4286, Umuarama, PR 87502-210, Brazil. E-mail: eduardoaraujo@unipar.br The behavior of the gill epithelium of tilapias cultured in tanks at different altitudes and interconnected with PVC pipes was analyzed. Gill filaments of four specimens from four tanks (T1, T2, T3 e T4) sequentially interconnected were submitted to histological routine to obtain 5-mm-thick cuts that were stained with HE or submitted to histochemistry reactions PAS + diastase solution or Alcian Blue pH 2.5 or Alcian Blue pH 1.0. Considering the intermediary, apical and basal regions of the filaments, the lamellar area was measured and the amount of mucous cells was counted. It was verified that oxygen, pH, and temperature decreased progressively as the water flew from one tank to another. Thus, an increase was realized of the amount of mucous cells and the lamellar area in T2, as well as a progressive decrease of these measures on the tanks which received water from T2. Moreover, detachment of the gill epithelium, cellular hyperplasia in the interlamellar space, and telangectasias were observed in the fishes from T2, T3 e T4. It was concluded that the aquatic environment in tanks sequentially interconnected with PVC pipes suffers alterations from one tank to another, as that physico-chemical fluctuations reflect on the behavior of the gill epithelium through variations of the lamellar area and the amount of mucous cells.

Abstract in English:

ABSTRACT.- Silverio S.M., Mari R.B, Clebis N.K., Scoz J.R., Germano R.M., Major J.A.A., Bombonato P.P. & Stabille S.R. 2009. The effects of ascorbic acid supplementation in ileum myenteric neurons of streptozotocin-induced diabetic rats. Pesquisa Veterinária Brasileira 29(4):295-302. Departamento de Cirurgia, Setor de Anatomia dos Animais Domésticos e Silvestres, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-270, Brazil. E-mail: remari@usp.br The exacerbation of the oxidative stress and of the polyol pathway which impair damage myenteric plexus are metabolic characteristics of diabetes. The ascorbic acid (AA) is an antioxidant and an aldose reductase inhibitor, which may act as neuroprotector. The effects of AA supplementation on the density and cellular body profile area (CP) of myenteric neurons in STZ-induced diabetes in rats were assessed. Four groups with five animals each were formed: normoglycemic (C); diabetic (D); AA-treated diabetic (DS) and AA-treated normoglycemic (CS). Dosagen of 50mg of AA were given, three times a week, for each animal (group DS and CS). Ninety days later and after euthanasia, the ileum was collected and processed for the NADPH-diaphorase technique. There were no differences (P>0.05) in the neuronal density among the groups. The CP area was lower (P<0.05) in the DS and CS groups, with a higher incidence of neurons with a CP area exceeding 200mm2 for groups C and D. The AA had no influence on the neuronal density in the ileum but had a neuroprotective effect, preventing the increase in the CP area and allowing a higher number of neurons with a CP area with less than 200mm2.

• Vitamin C diabetes intestine myenteric plexus

Abstract in Portuguese:

ABSTRACT.- Silverio S.M., Mari R.B, Clebis N.K., Scoz J.R., Germano R.M., Major J.A.A., Bombonato P.P. & Stabille S.R. 2009. The effects of ascorbic acid supplementation in ileum myenteric neurons of streptozotocin-induced diabetic rats. Pesquisa Veterinária Brasileira 29(4):295-302. Departamento de Cirurgia, Setor de Anatomia dos Animais Domésticos e Silvestres, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo, SP 05508-270, Brazil. E-mail: remari@usp.br The exacerbation of the oxidative stress and of the polyol pathway which impair damage myenteric plexus are metabolic characteristics of diabetes. The ascorbic acid (AA) is an antioxidant and an aldose reductase inhibitor, which may act as neuroprotector. The effects of AA supplementation on the density and cellular body profile area (CP) of myenteric neurons in STZ-induced diabetes in rats were assessed. Four groups with five animals each were formed: normoglycemic (C); diabetic (D); AA-treated diabetic (DS) and AA-treated normoglycemic (CS). Dosagen of 50mg of AA were given, three times a week, for each animal (group DS and CS). Ninety days later and after euthanasia, the ileum was collected and processed for the NADPH-diaphorase technique. There were no differences (P>0.05) in the neuronal density among the groups. The CP area was lower (P<0.05) in the DS and CS groups, with a higher incidence of neurons with a CP area exceeding 200mm2 for groups C and D. The AA had no influence on the neuronal density in the ileum but had a neuroprotective effect, preventing the increase in the CP area and allowing a higher number of neurons with a CP area with less than 200mm2.

Abstract in English:

ABSTRACT.- Silva C.A.O., Perri S.H.V., Koivisto M.B., Silva A.M., Carvalho R.G. & Monteiro C.M.R. 2009. [Histological and morphometric evaluation of the testes of cats (Felis catus).] Aspectos histológicos e morfométricos dos testículos de gatos domésticos (Felis catus). Pesquisa Veterinária Brasileira 29(4):312-316. Curso de Medicina Veterinária, Faculdade de Odontologia, Universidade Estadual Paulista, Rua Clóvis Pestana 793, Araçatuba, SP 16050-680, Brazil. E-mail: monteiro@fmva.unesp.br This paper deals with a comparative histologic and morphometric study of the testes of domestic cats distributed into two groups: Group 1, cats until 1 year of age, and Group 2, cats over 1 year. It was found that: (1) at 4 months of age the seminiferous tubules were poorly developed, appeared as seminiferous cords without lumen, lined by a low epithelium, and showed undifferentiated Sertoli cells and scarce interstitial tissue; (2) at 5 months the seminiferous tubules began to differentiate with increase in tubular diameter and lumen, the other tubular structures remaining similar to those previous referred; (3) at 6 and 7 months of age spermatocytogenesis began to appear, Leydig cells were large, polyhedral in shape, with vacuolated cytoplasm and clear nuclei, resting on a sparse interstitial tissue with few blood vessels; (4) 1-year-old cats showed testicular histological features of an adult animal, had seminiferous tubules of large diameter and high seminiferous epithelium with small lumen, and Leydig cells of different sizes, with polyhedral shape, vacuolated cytoplasm, clear nuclei and evident nucleoli resting in a sparse interstitial tissue with some blood vessels; (5) in Group 1 the average diameter of the seminiferous tubules was 160.58µm, and 185.94µm in Group 2; (6) the height of the seminiferous epithelium was 49.51µm for Group 1 and 63.29µm for Group 2; (7) the largest measures of the analyzed parameters were found in animals of Group 2, with functional reproductive organs.

• Histology morphometry testes cats

Abstract in Portuguese:

ABSTRACT.- Silva C.A.O., Perri S.H.V., Koivisto M.B., Silva A.M., Carvalho R.G. & Monteiro C.M.R. 2009. [Histological and morphometric evaluation of the testes of cats (Felis catus).] Aspectos histológicos e morfométricos dos testículos de gatos domésticos (Felis catus). Pesquisa Veterinária Brasileira 29(4):312-316. Curso de Medicina Veterinária, Faculdade de Odontologia, Universidade Estadual Paulista, Rua Clóvis Pestana 793, Araçatuba, SP 16050-680, Brazil. E-mail: monteiro@fmva.unesp.br This paper deals with a comparative histologic and morphometric study of the testes of domestic cats distributed into two groups: Group 1, cats until 1 year of age, and Group 2, cats over 1 year. It was found that: (1) at 4 months of age the seminiferous tubules were poorly developed, appeared as seminiferous cords without lumen, lined by a low epithelium, and showed undifferentiated Sertoli cells and scarce interstitial tissue; (2) at 5 months the seminiferous tubules began to differentiate with increase in tubular diameter and lumen, the other tubular structures remaining similar to those previous referred; (3) at 6 and 7 months of age spermatocytogenesis began to appear, Leydig cells were large, polyhedral in shape, with vacuolated cytoplasm and clear nuclei, resting on a sparse interstitial tissue with few blood vessels; (4) 1-year-old cats showed testicular histological features of an adult animal, had seminiferous tubules of large diameter and high seminiferous epithelium with small lumen, and Leydig cells of different sizes, with polyhedral shape, vacuolated cytoplasm, clear nuclei and evident nucleoli resting in a sparse interstitial tissue with some blood vessels; (5) in Group 1 the average diameter of the seminiferous tubules was 160.58µm, and 185.94µm in Group 2; (6) the height of the seminiferous epithelium was 49.51µm for Group 1 and 63.29µm for Group 2; (7) the largest measures of the analyzed parameters were found in animals of Group 2, with functional reproductive organs.