Resultado da pesquisa (14)

Termo utilizado na pesquisa mycosis

#11 - Outbreaks of rhinofacial and rhinopharyngeal zygomycosis in sheep in Paraíba, northeastern Brazil, p.29-35

Abstract in English:

Abstract.- Riet-Correa F., Dantas A.F.M., Azevedo E.O., Simões S.D.V., Silva S.M.S., Vilela R. & Mendoza L. 2008. Outbreaks of rhinofacial and rhinopharyngeal zygomycosis in sheep in Paraíba, northeastern Brazil. Pesquisa Veterinária Brasileira 28(1):29-35. Hospital Veterinário, CSTR, Universidades Federal de Campina Grande, Patos, PB 58700-000, Brazil. E-mail: franklin.riet@pq.cnpq.br Two outbreaks of zigomycosis with rhinofacial and two other with rhinopharyngeal lesions involving fungi with filamentous coaenocytic hyphae characteristic of entomoph-thoramycetous fungi are reported in the state of Paraíba, northeastern Brazil. One outbreak of rhinofacial zygomycosis occurred during the rainy season affecting 5 sheep. Another outbreak of the clinical form affected one out of 40 sheep during the dry season. Common clinical signs of the rhinofacial infection were bilateral serosanguineous nasal discharge with swelling of nostrils, upper lip, and the skin of the face. At necropsy the nasal mucosa showed dark brownish ulcerated areas which extended from the mucocutaneous region to 10cm inside the nasal vestibule. The mucosa of the hard palate was also ulcerated. The cutting surface of nostrils and palate showed a brownish or red spongeous tissue of friable consistency. One outbreak of rhinopharyngitis took place on an irrigated coconut farm; 7 out of 60 adult sheep were affected. Another outbreak affected a sheep in a flock of 80 during the dry season. Clinical signs as noisy respiration and dyspnoea due to mechanical blockage of the nasal cavities, swelling of the nostrils, and serosanguineous nasal discharge were observed. Six out of 8 sheep in this group showed exophthalmia, keratitis and unilateral corneal ulceration of the eye. The sheep either died of their infection or were euthanized after a clinical course of 7-30 days. At necropsy there was a dense yellow exudate in the nasopharyngeal area affecting the ethmoidal region, turbinate bones, paranasal sinuses, hard and soft palates, orbital cavity, pharynges, regional muscles and lymph nodes. Histopathologically both forms of the disease showed multifocal granulomas with an eosinophilic necrotic reaction (Splendore-Hoeppli phenomenon) containing ribbon type coenocytic hyphae with 7-30mm in diameter similar to hyphae of zygomycetous fungi, possibly Conidiobolus spp. Outbreaks of both forms of mycotic rhinitis are common in northeastern Brazil and in other regions of the country.

Abstract in Portuguese:

Abstract.- Riet-Correa F., Dantas A.F.M., Azevedo E.O., Simões S.D.V., Silva S.M.S., Vilela R. & Mendoza L. 2008. Outbreaks of rhinofacial and rhinopharyngeal zygomycosis in sheep in Paraíba, northeastern Brazil. Pesquisa Veterinária Brasileira 28(1):29-35. Hospital Veterinário, CSTR, Universidades Federal de Campina Grande, Patos, PB 58700-000, Brazil. E-mail: franklin.riet@pq.cnpq.br Two outbreaks of zigomycosis with rhinofacial and two other with rhinopharyngeal lesions involving fungi with filamentous coaenocytic hyphae characteristic of entomoph-thoramycetous fungi are reported in the state of Paraíba, northeastern Brazil. One outbreak of rhinofacial zygomycosis occurred during the rainy season affecting 5 sheep. Another outbreak of the clinical form affected one out of 40 sheep during the dry season. Common clinical signs of the rhinofacial infection were bilateral serosanguineous nasal discharge with swelling of nostrils, upper lip, and the skin of the face. At necropsy the nasal mucosa showed dark brownish ulcerated areas which extended from the mucocutaneous region to 10cm inside the nasal vestibule. The mucosa of the hard palate was also ulcerated. The cutting surface of nostrils and palate showed a brownish or red spongeous tissue of friable consistency. One outbreak of rhinopharyngitis took place on an irrigated coconut farm; 7 out of 60 adult sheep were affected. Another outbreak affected a sheep in a flock of 80 during the dry season. Clinical signs as noisy respiration and dyspnoea due to mechanical blockage of the nasal cavities, swelling of the nostrils, and serosanguineous nasal discharge were observed. Six out of 8 sheep in this group showed exophthalmia, keratitis and unilateral corneal ulceration of the eye. The sheep either died of their infection or were euthanized after a clinical course of 7-30 days. At necropsy there was a dense yellow exudate in the nasopharyngeal area affecting the ethmoidal region, turbinate bones, paranasal sinuses, hard and soft palates, orbital cavity, pharynges, regional muscles and lymph nodes. Histopathologically both forms of the disease showed multifocal granulomas with an eosinophilic necrotic reaction (Splendore-Hoeppli phenomenon) containing ribbon type coenocytic hyphae with 7-30mm in diameter similar to hyphae of zygomycetous fungi, possibly Conidiobolus spp. Outbreaks of both forms of mycotic rhinitis are common in northeastern Brazil and in other regions of the country.


#12 - Conidiobolomicose em ovinos no Estado de Mato Grosso, p.77-81

Abstract in English:

ABSTRACT.- Boabaid F.M., Ferreira E.V., Arruda L.P., Gasparetto N.D., Souza R.L., Silva M.C., Dutra V., Nakazato L. & Colodel E.M. 2008. [Conidiobolomycosis in sheep in the state of Mato Grosso, Brazil.] Conidiobolomicose em ovinos no Estado de Mato Grosso. Pesquisa Veterinária Brasileira 28(1):77-81. Departamento de Clínica Médica Veterinária, Faculdade de Agronomia e Medicina Veterinária, Universidade Federal de Mato Grosso, Av. Fernando Correia da Costa s/n, Bairro Coxipó, Cuiabá, MT 78068-900, Brasil. E-mail: moleta@ufmt.br The epidemiological, clinical, pathological and mycological findings of an outbreak of conidiobolomycosis in a flock of 40 Santa Ines sheep, in the state of Mato Grosso, Brazil, are reported. The illness occurred in the municipality of Nobres during January-June, 2007, resulting in death of about 30% of the affected sheep within 2-5 weeks. The clinical signs were characterized by apathy, weight loss, labored and noisy breathing with dyspnea, and mucous or serohemorrhagic nasal discharge. In three sheep there was unilateral exophthalmia, keratitis and corneal ulceration. A firm whitish, multi-lobulated, friable growth was evident in the ethmoturbinate region at the coronal sections of the head from six affected sheep. In all sheep the choana had similar nodular infiltration which resulted in local obstruction. In three of those there was exophthalmia. There was infiltration in the cribiform plate and brain (2 cases), regional lymph nodes (2), lungs (3), and abomasums (1). Microscopic findings were granulomatous inflammation of the rhinoetimoidal region, with necrosis, lymphocytic infiltration, epithelioid multinucleated giant cells and fibrovascular tissue, surrounding Splendore-Hoeppli material wich contained unstained ghost images of hyphae. The methenamine-silver stain uncover fungi hyphae, rarely ramified with bulbous dilatation in their extremities. Conidiobolus sp. was isolated from nasal tissue lesions of four sheep.

Abstract in Portuguese:

ABSTRACT.- Boabaid F.M., Ferreira E.V., Arruda L.P., Gasparetto N.D., Souza R.L., Silva M.C., Dutra V., Nakazato L. & Colodel E.M. 2008. [Conidiobolomycosis in sheep in the state of Mato Grosso, Brazil.] Conidiobolomicose em ovinos no Estado de Mato Grosso. Pesquisa Veterinária Brasileira 28(1):77-81. Departamento de Clínica Médica Veterinária, Faculdade de Agronomia e Medicina Veterinária, Universidade Federal de Mato Grosso, Av. Fernando Correia da Costa s/n, Bairro Coxipó, Cuiabá, MT 78068-900, Brasil. E-mail: moleta@ufmt.br The epidemiological, clinical, pathological and mycological findings of an outbreak of conidiobolomycosis in a flock of 40 Santa Ines sheep, in the state of Mato Grosso, Brazil, are reported. The illness occurred in the municipality of Nobres during January-June, 2007, resulting in death of about 30% of the affected sheep within 2-5 weeks. The clinical signs were characterized by apathy, weight loss, labored and noisy breathing with dyspnea, and mucous or serohemorrhagic nasal discharge. In three sheep there was unilateral exophthalmia, keratitis and corneal ulceration. A firm whitish, multi-lobulated, friable growth was evident in the ethmoturbinate region at the coronal sections of the head from six affected sheep. In all sheep the choana had similar nodular infiltration which resulted in local obstruction. In three of those there was exophthalmia. There was infiltration in the cribiform plate and brain (2 cases), regional lymph nodes (2), lungs (3), and abomasums (1). Microscopic findings were granulomatous inflammation of the rhinoetimoidal region, with necrosis, lymphocytic infiltration, epithelioid multinucleated giant cells and fibrovascular tissue, surrounding Splendore-Hoeppli material wich contained unstained ghost images of hyphae. The methenamine-silver stain uncover fungi hyphae, rarely ramified with bulbous dilatation in their extremities. Conidiobolus sp. was isolated from nasal tissue lesions of four sheep.


#13 - Epidemiologia e sinais clínicos da conidiobolomicose em ovinos no Estado do Piauí, p.184-190

Abstract in English:

ABSTRACT.- Silva S.M.M.S., Castro R.S., Costa F.A.L., Vasconcelos A.C., Batista M.C.S., Riet-Correa F. & Carvalho E.M.S. 2007. [Epidemiology and symptoms of conidiobolomycosis in sheep in the State of Piauí, Brazil.] Epidemiologia e sinais clínicos da conidiobolomicose em ovinos no Estado do Piauí. Pesquisa Veterinária Brasileira 27(4):184-190. Departamento de Clínica e Cirurgia, Centro de Ciências Agrárias, Universidade Federal do Piauí, Campus da Socopo s/n, Teresina, PI 64049-550, Brazil. E-mail: silvanammss@ufpi.br Conidiobolomycosis is reported in 25 farms, from January 2002 to December 2004, in the state of Piauí. The disease affects only sheep, mainly in April-June. The mean morbidity rate was 2.80%, but was higher in the first semester (2.1%), during the raining period, than in the second one (0.69%), during the dry period. Morbidity rate among flocks varied from 0.1-14.3%. Case fatality rate was 100%, and the clinical manifestation period varied from 1-5 weeks. Clinical signs were serous, mucous and/or bloody nasal secretion, respiratory distress, snoring respiration, cranium-facial asymmetry, exophthalmia, fever and progressive emaciation. Marked depression, sometimes with the head down or head pressing was observed in some cases. Gross, microscopic and ultrastructural lesions and identification of the agent are reported elsewhere. This is the first report of conidiobolomycosis in Brazil, which is endemic and has a high frequency in sheep in the State of Piauí, associated with high rainfalls (1000-1600mm annually) and high temperature (19-36°C).

Abstract in Portuguese:

ABSTRACT.- Silva S.M.M.S., Castro R.S., Costa F.A.L., Vasconcelos A.C., Batista M.C.S., Riet-Correa F. & Carvalho E.M.S. 2007. [Epidemiology and symptoms of conidiobolomycosis in sheep in the State of Piauí, Brazil.] Epidemiologia e sinais clínicos da conidiobolomicose em ovinos no Estado do Piauí. Pesquisa Veterinária Brasileira 27(4):184-190. Departamento de Clínica e Cirurgia, Centro de Ciências Agrárias, Universidade Federal do Piauí, Campus da Socopo s/n, Teresina, PI 64049-550, Brazil. E-mail: silvanammss@ufpi.br Conidiobolomycosis is reported in 25 farms, from January 2002 to December 2004, in the state of Piauí. The disease affects only sheep, mainly in April-June. The mean morbidity rate was 2.80%, but was higher in the first semester (2.1%), during the raining period, than in the second one (0.69%), during the dry period. Morbidity rate among flocks varied from 0.1-14.3%. Case fatality rate was 100%, and the clinical manifestation period varied from 1-5 weeks. Clinical signs were serous, mucous and/or bloody nasal secretion, respiratory distress, snoring respiration, cranium-facial asymmetry, exophthalmia, fever and progressive emaciation. Marked depression, sometimes with the head down or head pressing was observed in some cases. Gross, microscopic and ultrastructural lesions and identification of the agent are reported elsewhere. This is the first report of conidiobolomycosis in Brazil, which is endemic and has a high frequency in sheep in the State of Piauí, associated with high rainfalls (1000-1600mm annually) and high temperature (19-36°C).


#14 - Histochemical diagnosis of actinomycotic-like granulomas in cattle from southern Brazil

Abstract in English:

The etiology of actinomycotic-like lesions from 254 lymph nodes of cattle from southern Brazil was determined by histochemistry. The diagnosis of these lesions in hematoxylin-eosin (H-E) preparations was based upon the pyogranulomatous inflammatory reaction with centrally located sulphur grains. The lesions stained by the MacCallum-Goodpasture technique showed 60.36% Gram-positive coccoid bacteria (Staphylococcus), 36.62% Gram-negative cocco-bacillus (Actinobacillus lignieresz) and 2.72% Gram-positive filamentous branching elements (Actinomyces bovis). Fite-Faraco stain demonstrated the acid-fast properties and Gomori stain, the morphological features to distinguish between Actinomyces bovis .and Nocardia sp. Gram-positive cocei were diagnosed as botryomycosis which occurred predominantly in prescapular, parotid and femoral lymph nodes. The presence of Gram-negative coccobacillary elements indicated Actinóbacillus sp., whereas the finding of Gram-positive filamentous branching elements indicated Actinomyces sp. These latter two were more frequently found in retropharyngeal and sublingual lymph nodes. Small differences in the morphology of the bacterial colonies may help to establish the etiological diagnosis in H-E preparations. The Gram method, however, allows an accurate distinction between Actinobacillus lignieresi and Actinomyces bovis in cases where fresh material for bacteriological isolation is no longer available. Of 467 other cases originally diagnosed as tuberculosis in gross inspection at the slaughter house, 10.23% were histologically confirmed as actinomycotic-like granulomas. This certainly causes economic losses due to the condemnation of carcasses.

Abstract in Portuguese:

Foi determinada a etiologia dos "granulomas actinomicóides" através da histoquímica em 254 materiais de linfonodos de bovinos do Rio Grande do Sul. O diagnóstico de granuloma actinomicóide foi baseado na inflamação piogranulomatosa, contendo, na porção central, os grânulos de enxofre, observados na coloração pela hematoxilina e eosina (H-E). Ao material com essa lesão foi aplicada a técnica de MacCallum-Ooodpasture que evidenciou uma incidência de 60,36% de bactérias cocoides Gram-positivas (Staphylococcus), 36,62% de coco-bacilos Gram-negativos (Actinobacillus lignieresz) e de 2,72% de elementos filamentosos, ramificados, Gram-positivos (Actinomyces bovis) como constituintes do grão de enxofre. Nos casos de actinomicose aplicaram-se as técnicas de Fite-Faraco e Prata de Gomori para a diferenciação entre Actinomyces bovis e Nocardia sp. Os casos de botriomicose foram vistos principalmente nos linfonodos pré-escapulares, parotídeos e femurais; os de actinobacilose e actinomicose nos retrofaringes e sublinguais. Pequenas diferenças do aspecto das colônias podem auxiliar no diagnóstico etiológico nas preparações coradas pela H-E, mas o emprego das técnicas de Gram permite a diferenciação entre Actinobacillus lignieresi e Actinomyces bovis quando não se dispõe de culturas. Adicionalmente observou-se pelo estudo histológico de outros 467 casos, que 10,23% deles foram diagnosticados como granuloma actinomicóide, porém interpretados macroscopicamente, na inspeção, como tuberculose o que certamente leva a prejuízos econômicos pela condenação das carcaças.


Colégio Brasileiro de Patologia Animal SciELO Brasil CAPES CNPQ UNB UFRRJ CFMV