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Year 2006 - Volume 26, Number 1
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Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protocols and media
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Ristow P., Silva M.G., Fonseca L.S. & Lilenbaum W. 2006. [Evaluation of Mycobac-terium avium subsp. paratuberculosis faecal culture protocols and media.] Pesquisa Veteri-nária Brasileira 26(1):1-4. Mycobacteria Laboratory, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, RJ 21941-590, Brazil. E-mail: paularistow@bigfoot.com
Paratuberculosis is an important enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease is officially considered exotic in Brazil, but recent serological surveys and the isolation of the agent suggest it may occur in our herds. The aim of this study was to evaluate three different formulations of Herrold’s egg yolk agar with mycobactin J (HEYM) and four faecal culture protocols considering their ability for Map growth as well as cost and ease of application. Three formulations of HEYM were inoculated with two suspensions of Map. Spiked faeces and naturally contaminated faecal samples were treated by the four faecal culture protocols. Centrifugation protocol and HEYM recommended by OIE showed the best results on the recovery of Map. |
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