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Year 2010 - Volume 30, Number 10
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Cortisol influence on testicular testosterone secretion in domestic cat: An in vitro study, 30(10):887-890
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ABSTRACT.- Genaro G. & Franci C.R. 2010. Cortisol influence on testicular testosterone secretion in domestic cat: An in vitro study. [Ação do cortisol sobre a secreção de testosterona em testículos de gato doméstico: um estudo in vitro.] Pesquisa Veterinária Brasileira 30(10):887-890. Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Cx. Postal 390, Ribeirão Preto, SP 14001-970, Brazil. E-mail: ggenaro@ffclrp.usp.br
The aim of the present experiment was to investigate the effect of corticosteroids (exogen) on in vitro testosterone secretion after stress by transportation (40 minutes). Feline testes (Felis silvestris catus) were incubated in the following media: TCM 199; TCM 199 + hCG 10–7M; TCM 199 + hydrocortisone 10–7M, or TCM 199 + hCG + hydrocortisone. The animals (n=21) were allocated into three groups: (S) that arrived at 3 h prior to surgery, (A) that remained in the laboratory for 36 h before being submitted to surgical procedure, and (C) that were also allowed to remain for 36 hours in the laboratory before the surgical procedure, but whose testes had been incubated with hydrocortisone prior to incubation in the referred media. The results showed that group S secreted higher levels of testosterone, regardless of the culture media. It is noteworthy that the suppressing action of hydrocortisone sodium succinate led to a reduction in the testosterone concentration, despite the presence of hCG. |
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