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Year 2019 - Volume 39, Number 1
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Effects of atracurium besylate on corneal endothelium of chickens: in vitro study, 39(1):70-74
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ABSTRACT.- Guimarães C.B., Albuquerque L.,Torikachvili M., Vargas E.V., Dall’Agnol C.C., Silva T.C. & Pigatto J.A.T. 2019. Effects of atracurium besylate on corneal endothelium of chickens: in vitro study. Pesquisa Veterinária Brasileira 39(1):70-74. Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9090, Agronomia, Porto Alegre, RS 91540-000, Brazil. E-mail: pigatto@ufrgs.br
The aim of this study was to investigate the acute effects of atracurium besylate on cellular damage in corneal endothelium of chickens. Twenty healthy chicken eyes were assigned to the following groups: Group 1 (G1), experimental group (n=10); and Group 2 (G2), control (n=10). Excised corneoscleral buttons were immediately placed on glass microscopy slides with endothelial region faced up. Corneal endothelium of eyes in G1 were covered with AB (0.2mL, 10mg/mL) for 3 min and then rinsed with balanced salt solution (BSS), while the corneal endothelium of eyes in G2 were covered with BBS for 3 min. Corneas from both groups were stained with alizarin red/trypan blue and visualized by light microscopy. Ten random photographs were taken from each cornea. The area of cellular damage was measured by software in all samples and cell loss of each group was averaged and compared. Endothelial area of denudation and Descemet’s membrane exposure were higher in G1 than G2. In conclusion, atracurium besylate induced an acute damage on corneal endothelium of chickens. |
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