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Year 2018 - Volume 38, Number 11
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Frequency of Staphylococcus aureus virulence genes in milk of cows and goats with mastitis, 38(11):2029-2036
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ABSTRACT.- Acosta A.C., Oliveira P.R.F., Albuquerque L., Silva I.F., Medeiros E.S., Costa M.M., Pinheiro Junior J.W. & Mota R.A. 2018. Frequency of Staphylococcus aureus virulence genes in milk of cows and goats with mastites. [Frequência de genes fatores de virulência de Staphylococcus aureus em leite de vacas e cabras com mastite.] Pesquisa Veterinária Brasileira 38(11):2029-2036. Laboratório de Bacterioses dos Animais Domésticos, Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros s/n, Dois Irmãos, Recife, PE 52171-900, Brazil. E-mail: acabad80@gmail.com
The present study determined the frequency of Staphylococcus aureus virulence genes in 2,253 milk samples of cows (n=1000) and goats (n=1253) raised in three different geographical regions of the state Pernambuco, Brazil. The presence of genes of virulence factors associated to adhesion to host cells (fnbA, fnbB, clfA and clfB), toxinosis (sea, seb, sec, sed, seg, seh, sei, tsst, hla and hlb), and capsular polysaccharide (cap5 and cap8) was evaluated by PCR. A total of 123 and 27 S. aureus strains were isolated from cows’ and goats’ milk, respectively. The sec and tsst genes were detected exclusively in goats’ isolates, while the seh gene was only identified in cows’ isolates. The number of toxin genes per strain showed that goats’ isolates are likely more toxic than bovines’ isolates. The cap5 genotype predominated in both host species, especially in strains collected from cows raised in the Agreste region. The cap8 genotype is likely more virulent due to the number of virulence genes per strain. The results of the present study demonstrate that S. aureus may pose a potential threat to human health in Brazil, and, therefore, these results should support actions related to mastitis control programs. |
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