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Year 2018 - Volume 38, Number 6
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Virulence and multiplication of Toxoplasma gondii isolates from the central region of Rio Grande do Sul, Brazil, 38(6):1026-1029
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ABSTRACT.- Cadore G.C., Camillo G., Sangioni L.A. & Vogel F.S.F. 2018. [Virulence and multiplication of Toxoplasma gondii isolates from the central region of Rio Grande do Sul, Brazil.] Virulência e multiplicação de isolados de Toxoplasma gondii da região central do Rio Grande do Sul. Pesquisa Veterinária Brasileira 38(6):1026-1029. Laboratório de Doenças Parasitárias, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Santa Maria, Av. Roraima 1000, Camobi, Santa Maria, RS 97105-900, Brazil. E-mail: fefevogel@gmail.com
The protozoan Toxoplasma gondii has the ability to infect several animal species, usually causing reproductive disorders. Four different isolates of T. gondii - Pains #1 (P1), Pains #2 (P2), Santa Flora #1 (SF1) and Santa Flora #306 (SF306) were evaluated with prior genotyping. Their virulence and multiplication capacity were analyzed in vitro (Vero cells) and in vivo (mice). For each isolate, three passages were performed with dose inoculation 1x104 tachyzoites at each passage. The mice were daily observed to verify of clinical signs and occurrence of mortality after tachyzoites inoculation. The SF1 isolate and SF306 isolate, presented the highest multiplication of the total tachyzoites number in each of the 3 different passages performed in vitro and in vivo. The initial clinical signs in mice were observed between 5 and 11 days, and occurring mortality between 6 and 15 days, after inoculation. Thus, the parasitic multiplication of theses isolates is similar in vitro and in vivo; different isolates within the same genotype have a similar virulence and the SF1 isolate is the most virulent for mice. |
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