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Year 2017 - Volume 37, Number 3
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Comparative study of double spin and use of E-PET filter (Equine Platelet Enhancement Therapy) to obtain platelet rich plasma in horses, 37(3):215-220
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ABSTRACT.- Conceição M.L., Alvarenga M.L., Souza J.B., Mattos L.H.L., Rodrigues C.A., Watanabe M.J., Hussni C.A. & Alves A.L.G. 2017. [Comparative study of double spin and use of E-PET filter (Equine Platelet Enhancement Therapy) to obtain platelet rich plasma in horses.] Plasma rico em plaquetas: estudo comparativo entre a obtenção através da dupla centrifugação e filtro E-PET (Equine Platelet Enchancement Therapy). Pesquisa Veterinária Brasileira 37(3):215-220. Departamento de Cirurgia e Anestesiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Campus de Botucatu, Distrito de Rubião Júnior s/n, Botucatu, SP 18618-970, Brazil. E-mail: anaalves@fmvz.unesp.br
The platelet-rich plasma (PRP) consists of a high concentration of platelets in a small volume of plasma, four times greater (average) than the serum concentration. The use of PRP is justified by the high concentration of growth factors present in granules in the platelets, which have several functions such as cell proliferation, chemotaxis, angiogenesis and differenciation, which extend the power of tissue repair. There are several protocols to obtain PRP in horses described in the literature, among which are highlighted the double centrifugation, automated and filters. There are substantial differences in the PRP content depending on its method of production, especially when it concerns the amount of leukocytes, platelets and concentration of growth factors. Thus, the aim of this study was to compare the use of platelet concentrates obtained by double centrifugation protocol and obtained by the filter E-PET (Equine Platelet Enhancement Therapy) taking into account the platelet and leukocyte final concentration, the quantification of growth factors (TGFβ and PDGF-BB) and the facility to do those methods. Nine horses were used to obtain PRP by double centrifugation and through the E-PET filter, with no statistical difference (p>0.05) between the methods relative to the platelet and leukocyte concentration; however, there was statistical difference (p=0.002 and p=0.004 respectively) compared with the concentration of TGFβ and PDGF-BB. It was concluded that the E-PET filter proved to be a more effective method possible to use in the field and to provide a higher concentration of growth factors (TGFβ and PDGF-BB). |
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