Year 2017 - Volume 37, Number 1


Title
Evaluation of the concentration of allergens from mites in fur and households dust of dogs with atopic dermatitis, 37(1):41-46
Authors

Abstract
ABSTRACT.- Assunção D.L., Farias M.R., Barbosa M.C.R. & Machado L.H.A. 2017. Evaluation of the concentration of allergens from mites in fur and households dust of dogs with atopic dermatitis. Pesquisa Veterinária Brasileira 37(1):41-46. Mestrado em Ciência Animal, Escola de Ciências Agrárias e Medicina Veterinária, Pontifícia Universidade Católica do Paraná, BR-376 Km 14, São José dos Pinhais, PR 83010-500, Brazil. E-mail: devaki.la@hotmail.com

This study evaluated the concentration of Der p 1, Der f 1 and Blo t 5 in the fur and households of 20 dogs with atopic dermatitis (AD) and 20 healthy dogs. The diagnosis of AD was clinical based on Favrot’s criteria. Dust samples were collected with a domestic vacuum cleaner. For each site, 1m2 was vacuumed for 2 min. The samples were collected in separate filters, transferred into plastic containers, sealed and kept frozen until ELISA analysis. In the fur of atopic dogs the average concentration of Der p 1 was 0.25µg/g compared to 0.03µg/g in healthy dogs. In households with atopic dogs the highest concentrations of Der p 1 were found in carpets (2.18µg/g), followed by couches (1.53µg/g), beds (1.14µg/g), dogs’ bed linen (0.64µg/g) and floors (0.14µg/g). The concentrations of Der p 1 on carpets, couches and beds were significantly higher than in atopic dogs’ fur (p<0.05). There was no statistical difference when comparing the concentrations of Der f 1 and Blo t 5 in different environments of atopic dogs (p>0.05). The concentrations of Der p 1, Der f 1 and Blo t 5 were equivalent in atopic and non-atopic dog’s households. Among the allergens studied, Der p 1 was the most commonly found, predominantly in carpets and couches.
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