Year 2004 - Volume 24, Number 4


Title
Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs
Authors

Abstract
Minho A.P., Navarro I.T., Freire R.L., Vidotto O., Gennari S.M., Marana E.M. & Garcia J.L. 2004. Evaluation of the indirect fluorescent antibody test and modified agglutination test for detection of antibodies against Toxoplasma gondii in experimentally infected pigs. Pesquisa Veterinária Brasileira 24(4):199-202. Depto Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Cx. Postal 6001, Londrina, PR 86050-970, Brazil. E-mail: italmar@uel.br

The study determined the sensitivity and specificity of the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT) for anti-Toxoplasma gondii antibody detection by analyzing sera from 46 experimentally infected pigs. Values for sensitivity were 95.7% (confidence interval 95%: 84.0-99.2%) and for specificity 97.8% (confidence interval 95%: 87.0-99.9%) in both tests. There was an optimum agreement of results between IFAT and MAT evidenced by a Kappa test of 0.86. These results validate these tests for the detection of T. gondii infection in pigs. IFAT and MAT despite methodologies with different characteristics and readings have similar accuracy in pig serum samples.
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Colégio Brasileiro de Patologia Animal CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ISI Web of Knowledge SciELO - Scientific Electronic Library Online Banco de Dados Bibliográficos da USP UnB - Universidade de Brasília UFRRJ - Universidade Federal Rural do Rio de Janeiro CFMV - Conselho Federal de Medicina Veterinária