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Year 2019 - Volume 39, Number 7
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Subgingival bacterial microbiota associated with ovine periodontitis, 39(7):454-459
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ABSTRACT.- Silva N.S., Borsanelli A.C., Gaetti-Jardim Júnior E., Schweitzer C.M., Silveira J.A.S., Bomjardim H.A., Dutra I.S. & Barbosa J.D. 2019. Subgingival bacterial microbiota associated with ovine periodontitis. [Microbiota bacteriana subgengival associada à periodontite ovina.] Pesquisa Veterinária Brasileira 39(7):454-459. Laboratório de Patologia Clínica Veterinária, Faculdade de Medicina Veterinária, Instituto de Medicina Veterinária, Universidade Federal do Pará, Campus de Castanhal, Rodovia BR-316 Km 61, Castanhal, PA 68741-740, Brazil. E-mail: nataliasilvasilveira1@gmail.com
Periodontitis is an inflammatory response in a susceptible host caused by complex microbiota, predominantly composed of Gram-negative anaerobic bacteria. Aiming to characterize the subgingival bacterial microbiota associated with ovine periodontitis, polymerase chain reaction (PCR) was performed in subgingival periodontal pocket samples of 14 sheep with severe periodontitis and in subgingival sulcus biofilm of 14 periodontally healthy sheep in search mainly of Gram-negative and Gram-positive microorganisms considered important periodontopathogens. The most prevalent bacteria in the sheep with periodontal lesions were Tannerella forsythia (78.6%), Treponema denticola (78.6%), Fusobacterium nucleatum (64.3%), and Porphyromonas gingivalis (50%), whereas in the healthy sheep, F. nucleatum (42.8%) was the most often detected bacterium. Statistically significant differences were observed for Campylobacter rectus, Enterococcus faecium, Prevotella nigrescens, T. forsythia, and T. denticola (p<0.05) in the sheep with periodontitis in the comparison between groups. Aggregatibacter actinomycetemcomitans, Enterococcus faecalis, and Porphyromonas gulae were not detected in any of the samples analyzed. In conclusion, C. rectus, E. faecium, P. nigrescens, T. forsythia, and T. denticola were associated with severe lesions caused by ovine periodontitis, and F. nucleatum was the most prevalent microorganism in the subgengival sulcus biofilm of healthy sheep. |
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