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Year 1998 - Volume 18, Number 3
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Occurrence and distribution of Clostridium botulinum type C and D spores in buffalo breeding areas of the Baixada Maranhense, Maranhão, Brazil, 18(3/4):127-131
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ABSTRACT.- Silva T.M.D., Dutra I.S., Castro R.N. & Döbereiner J. 1998. [Occurrence and distribution of Clostridium botulinum type C and D spores in buffalo breeding areas of the Baixada Maranhense, Maranhão, Brazil.] Ocorrência e distribuição de esporos de Clostridium botulinum tipos C e D em áreas de criação de búfalos na Baixada Maranhense. Pesquisa Veterinária Brasileira 18(3/4):127-131. Depto Apoio, Produção e Saúde Animal, Unesp - Campus de Araçatuba, Cx. Postal 533, Araçatuba, SP 16015-050, Brazil.
As botulism is a common disease in butfaloes raised in the low lands of the State of Maranhão, Brazil, the occurrence of Clostridium botulinum spores was evaluated in butfalo breeding areas of 4 municipalities in the "Baixada Maranhense". Twenty eight samples of faeces, mud and soil were collected and divided into 140 subsamples, being 40 of faeces, 65 of mud and 35 of soil. Botulinum toxin was detected in the filtrates of 104 cultures (74.28%) from 140 subsantples through the inoculation of mice. Using the microcomplement fixation technique for the identification of C. botulinum toxins, type C (14.29%), D (82.14%) and CD complex (3.57%) were found. No significant ditferences (P>0.05%) between faeces, mud and soil samples were observed. There was a high contamination with C. botulinum spores of the butfalo faeces, mud and soil in the areas studied. Identification of other types and subtypes of C. botulinum was not attempted. |
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