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Year 1999 - Volume 19, Number 3
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Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate, 19(3/4):133-138
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ABSTRACT.- Fernández P.E., Sanguinetti H.R., Portiansky E.L, Barbeito C.G. & Gimeno E.J. 1999. Detection of illegal estrogen administration through immunohistochemical markers in the bovine prostate. [Verificação da administração ilegal de estrógenos através de marcadores imunohistoquímicos na próstata de bovinos] Pesquisa Veterinaria Brasileira 19(3/4):133-138. Institute of Pathology "Prof. Dr. Bernardo Epstein", School of Veterinary Sciences, National University of La Plata, P.O.Box 296, (1900) La Plata, Argentina.
The immunodetection of diverse cell markers was evaluated in prostatic samples from bullocks, and bullocks showing epithelial hyperplasia-metaplasia, with oestrogen-induced changes, and in experimental samples from bullocks inoculated with dietylstilbestrol (DES). Antigen-retrieval procedures allowed the use of tissues that had been fixed in formalin for long periods. Three tissue markers were chosen for the study: cytokeratins 13 and 16, vimentin and desmin. Monoclonal antibody K8.12 (specific for cytokeratins 13 and 16) stained basal cells and hyperplastic-metaplastic epithelium; monoclonal antivimentin, and desmin, allowed the definition of fibromuscular changes. |
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