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Year 2019 - Volume 39, Number 3
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Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil, 39(3):209-213
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ABSTRACT.- Rizotto L.S., Simão R.M., Scagion G.P., Simasaki A.A., Caserta L.C., Benassi J.C., Arns C.W. & Ferreira H.L. 2019. Detection of avian metapneumovirus subtype A from wild birds in the State of São Paulo, Brazil. Pesquisa Veterinária Brasileira 39(3):209-213. Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Av. Duque de Caxias Norte 225, Pirassununga, SP 13635-900, Brazil. E-mail: hlage@usp.br
The present study investigated the circulation of avian metapneumovirus (aMPV) in wild birds in Brazil. To do so, 131 samples from 366 oropharyngeal or cloacal swabs collected from 18 species of birds were tested individually or in pools by RT-PCR. Samples detected by RT-PCR were selected for DNA sequencing. Thirteen (9.9%) samples were detected by the RT-PCR targeting the N gene and four out of 13 samples were sequenced. Sequencing results showed a high identity with the aMPV subtype A. Our results confirm the circulation of the aMPV subtype A in wild birds in Brazil even five years after its last detection. |
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