Year 2018 - Volume 38, Number 10


Title
Morphological evaluation of corpus luteum for the diagnosis of 20-day pregnancy after IATF in crossbred cows., 38(10):2006-2011
Authors

Abstract
ABSTRACT.- Silva M.A.A., Rodrigues A.S., Brandão T.O., Maggitti Junior L.P., Carôso B.S.S., Chalhoub M., Bittencourt R.F. & Ribeiro Filho A.L. 2018. [Morphological evaluation of corpus luteum for the diagnosis of 20-day pregnancy after IATF in crossbred cows.] Avaliação morfofuncional do corpo lúteo para diagnóstico precoce de gestação 20 dias após IATF em vacas mestiças leiteiras. Pesquisa Veterinária Brasileira 38(10):2006-2011. Departamento de Anatomia, Patologia e Clínicas Veterinárias, Universidade Federal da Bahia, Rua Ademar de Barros 500, Ondina, Salvador, BA 40170-110, Brazil. E-mail: marianadeandradesilva@gmail.com

The super early gestation detection is of great relevance considering the increase of the herds’ productivity, in an attempt to reduce the time of service of these animals. Due to this relevance, this study aimed to use Doppler ultrasonography as a tool for gestation prediction, evaluating the luteal body (CL) irrigation twenty days after FTAI. 73 crossbred cows were evaluated 20 days after FTAI through trans rectal US. In order to obtain the diameter of the CL (DCL) and its area (ACL), B mode was used. The Color Doppler was applied to measure the​​ vascularization area (CLV) and its percentage in the CL (VCL %). The images were classified, based on the number of colored pixels and their distribution in CL, in two groups: negative predictive diagnosis of gestation (NPD) and positive predictive value (PPV). On the same day, LC functionality was determined based on P4 plasma concentration. The definitive gestation diagnosis was confirmed through US 35 days after the FTAIs. Student’s T test (with P<0.05) was used to evaluate the variables. Pregnant group presented higher averages (P<0.05) for all variables compared to the Non-Pregnant Group. The predictive diagnosis showed 83.33% of specificity, 100% of sensitivity and 91.79% of accuracy. The conclusion is that the US Doppler at 20 days post FTAI is a reliable method for early gestation diagnosis.
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