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Year 2018 - Volume 38, Number 5
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Cholesterol-loaded cyclodextrin on the cryopreservation of stallion sperm of the Northeastern breed, 38(5):991-996
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ABSTRACT.- Souza W.L., Moraes E.A. & Oliveira R.P. 2018. [Cholesterol-loaded cyclodextrin on the cryopreservation of stallion sperm of the Northeastern breed.] Colesterol carregado pela ciclodextrina sobre a criopreservação dos espermatozoides de garanhões da raça Nordestina. Pesquisa Veterinária Brasileira 38(5):991-996. Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Ceará, Avenida Dr. Silas Muguba 1700, Campus do Itaperi, Fortaleza, CE 60740-000, Brazil. E-mail: wilde@zootecnista.com.br
In the study effect of cholesterol was evaluated on the sperm quality of Nordestina stallion breed. Twenty semen samples were used from two stallions, diluted with BotuSemen extender and cholesterol add as follows: control, 0.75mg of cholesterol-loaded cyclodextrin (CLC) and 1.0mg of CLC/120x106 sperm/mL, and incubated for 15 min at 22°C. The samples were diluted 1:5 with lactose-yolk egg extender and cooled to 5°C over two hours, loaded into 0.5mL straws and frozen in static liquid nitrogen vapor before being plunged into nitrogen. Samples were thawed (37°C/30s) and analyzed. The variables were analyzed by ANOVA and means compared by Tukey test (P<0.05). Higher percentages of total and progressive motile was higher for sperm treated with CLC compared to control, and CLC promoted higher percentages (P<0.05) of total and progressive motility for the 3 hours of incubation. The percentage of viability and plasma membrane integrity of spermatozoa were higher (P<0.05) in sperm treated with CLC compared to the control group. The number of spermatozoa reacted to hypoosmotic test was higher (P<0.05) in sperm treated with CLC than control. Addition of CLC in the semen of Nordestina stallion breed improve the sperm quality after cryopreservation. |
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