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Year 2018 - Volume 38, Number 4
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Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases, 38(4):779-784
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ABSTRACT.- Di Filippo P.A., Lannes S.T., Meireles M.A.D., Nogueira A.F.S., Ribeiro L.M.F., Graça F.A.S. & Glória L.S. 2018. Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases. [Proteínas de fase aguda no soro e no líquido cefalorraquidiano de bovinos hígidos: possíveis usos na avaliação de doenças neurológicas.] Pesquisa Veterinária Brasileira 38(4):779-784. Laboratório de Clínicas e Cirurgia Veterinária, Centro de Ciências e Tecnologias Agropecuárias, Universidade Estadual do Norte Fluminense “Darcy Ribeiro”, Avenida Alberto Lamego 2000, Campos dos Goytacazes, RJ 28013-602, Brazil. E-mail: pdf@uenf.br
Use of acute-phase proteins (APPs) for assessment of health and disease in animals has increased greatly within the last decade. The objective was to determine the normal concentration of APPs in the serum and cerebrospinal fluid (CSF) of healthy cattle by polyacrylamide gel electrophoresis. Fifty crossbred animals (350±70kg of BW and 18±1.2 months of age), 25 heifers and 25 steers were used. CSF samples were collected from atlanto-occipital (AO) site and blood samples were obtained from the jugular vein. CSF and serum protein electrophoresis were performed by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Thirty-seven proteins with molecular weights ranging from 7 and 37kDa were identified in CSF of all animals. These eight were nominally identified with immunoglobulin A and G, celuloplasmin, transferrin, albumin, α1-antitripsin, acidic glycoprotein, and haptoglobin. All protein fractions in CSF did not differ between heifers and steers. In sera, 34 proteins with molecular weights between 7 and 244kDa were identified in heifers and steers. Similar proteins were nominally identified in the sera, but only the CSF presented α1-antitripsin. The serum values of acidic glycoprotein and immunoglobulin G were significantly higher in steers compared with heifers. In conclusion, measurement of CSF acute phase protein concentrations can be useful in diagnosing and monitoring the progression of bovine neurological diseases, perhaps even to guide therapeutic procedures. The CSF electrophoretic profile of healthy cattle does not change depending on gender. |
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