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Year 2018 - Volume 38, Number 3
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Influence of mastitis on electrophoretic fractions of colostrum from Holstein cows, 38(3):430-435
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ABSTRACT.- Santos G.G.F., Deschk M., Silva A.K.G., Poló T.S., Marinho M., Peiró J.R. & Feitosa F.L.F. 2018. [Influence of mastitis on electrophoretic fractions of colostrum from Holstein cows.] Influência da mastite nas frações eletroforéticas do colostro de vacas da raça Holandesa. Pesquisa Veterinária Brasileira 38(3):430-435. Departamento de Clínica, Cirurgia e Reprodução Animal, Faculdade de Medicina Veterinária de Araçatuba, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Rua Clóvis Pestana 793, Jardim Dona Amélia, Araçatuba, SP 16050-680, Brazil. E-mail: leydsonf@fmva.unesp.br
The aim of this study was to evaluate the protein fractions in colostral secretions of cows affected by mastitis immediately after calving. Therefore, 30 Holstein cows were divided into three groups: Group I (GI) composed of ten multiparous cows calving without mastitis; Group II (GII) composed of ten multiparous cows calving with subclinical mastitis, and Group III (GIII) composed of ten multiparous cows calving with mastitis. The concentration of immunoglobulin A (IgA), lactoferrin (LF), albumin, immunoglobulin G (IgG), β-lactoglobulin (β-Lg) and α-lactoalbumin (α-La) was determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). It was found that the IgG, LF and albumin vary among glands of subclinical and clinical mastitis and healthy and that the presence of a bacteria in the mammary gland was the key role for changing of the pattern of serum protein source. |
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