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Year 2018 - Volume 38, Number 1
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Quantification of IL-10 and IFN-γ in dogs with or without clinical signs of Leishmania (Leishmania) chagasi infection, 38(1):129-132
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ABSTRACT.- Rosário C.J.R.M., Dominici M.F., Braga M.S.C.B., Lima C.A.A., Pereira J.G. & Melo F.A. 2018. [Quantification of IL-10 and IFN-γ in dogs with or without clinical signs of Leishmania (Leishmania) chagasi infection.] Quantificação da IL-10 e do INF-γ em cães com ou sem sinais clínicos de infecção com Leishmania (Leishmania) chagasi. Pesquisa Veterinária Brasileira 38(1):129-132. Departamento de Patologia, Universidade Estadual do Maranhão, Cidade Universitária Paulo VI s/n, Tirirical, São Luís, MA 65055-000, Brazil. E-mail: carlajanaina_rm@hotmail.com
Leishmaniasis comprise a complex of diseases caused by intracellular mandatory parasites belonging to the genus Leishmania. Considered as an important public health problem, and domestic dogs are primarily responsible for maintaining the epidemiological chain of the disease, it is estimated that more than the half of the dogs infected do not show clinical signs of the disease. The profile of IL-10 and IFN-γ dogs naturally infected with Leishmania (Leishmania) chagasi in São Luís/MA was evaluated. Blood samples were collected from 50 animals, 20 from positive and symptomatic dogs for leishmaniasis canine (CVL), 20 from positive asymptomatic animals and 10 negative. Samples were analyzed by immunochromatographic test Dual Path Platform (DPP/Biomanguinhos®) and by indirect ELISA (EIE/Biomanguinhos®) for detection of anti-Leishmania antibodies. After the confirmation of the tests, the capture ELISA was performed for quantification of IL-10 and IFN-γ cytokines through the Milliplex MAP kit. There was a statistical difference between the groups, observing an increase of IL-10 in blood of symptomatic dogs for CVL, compared to the group of asymptomatic animals, suggesting that animals with this expression of IL-10 may be associated with susceptibility to disease. As well as the increase in IFN-γ levels in asymptomatic dogs, compared to the symptomatic dog group, could be related to chronicity of the disease. |
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