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Year 2017 - Volume 37, Number 1
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etection of immunoreactive proteins of Rickettsia sp. Atlantic Forest strain, 37(1):52-57
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ABSTRACT.- Oliveira C.S., Braunig P., Krawczak F., Labruna M.B., Botton S.A., Vogel F.S.F. & Sangioni L.A. 2017. [Detection of immunoreactive proteins of Rickettsia sp. Atlantic Forest strain.] Detecção de proteínas imunorreativas de Rickettsia sp. cepa Mata Atlântica. Pesquisa Veterinária Brasileira 37(1):52-57. Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria, Av. Roraima 1000, Prédio 44, Sala 5149, Santa Maria, RS 97105-900, Brazil. E-mail: carolsobotyk@gmail.com
Brazilian Spotted Fever (BSF) is an infectious disease transmitted by ticks to humans. A new human rickettsial infection was reported to cause spotted fever in the State of São Paulo and was named Rickettsia sp. Strain Atlantic Forest. This study aimed to detect and identify proteins with potential to stimulate the immune system of mammalian host of this new strain described. Therefore, we performed total protein extraction Rickettsia sp. Strain Atlantic Forest. The extracted proteins were fractionated by electrophoresis. The protein bands were transferred to nitrocellulose membrane by electrical migration and subjected to Western blot for protein detection. In all, seven immunoreactive proteins were detected. Two proteins showed higher abundance, with molecular weight of 200 and 130 kDa respectively. By comparing existing proteomic maps and the molecular weight of these proteins showed that, it is suggested that the two proteins detected representing rOmpA (200 kDa) and rOmpB (130 kDa). The other detected proteins had lower occurrence and molecular weight less than 78 kDa, which may represent members of the cell surface antigens Family (Sca - Surface cell antigen). The detected proteins may serve as a study based on the development of sensitive and specific diagnostic methods in the development of vaccines and they enable further studies to more effective therapies. |
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