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Year 2014 - Volume 34, Number 12
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Occurrence of tad genes associated with biofilm formation in isolates of Pasteurella multocida from lungs of pigs with pneumonia, 34(12):1147-1152
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ABSTRACT.- Moraes D.F.S.D., Brandão L.N.S., Pitchenin L.C., Filho J.X.O., Morés N., Nakazato L. & Dutra V. 2014. [Occurrence of tad genes associated with biofilm formation in isolates of Pasteurella multocida from lungs of pigs with pneumonia.] Ocorrência de genes tad associados à formação de biofilme em isolados de Pasteurella multocida de pulmões de suínos com pneumonia. Pesquisa Veterinária Brasileira 34(12):1147-1152. Laboratório de Microbiologia e Biologia Molecular, Universidade Federal do Mato Grosso, Av. Fernando Corrêa da Costa 2367, Bairro Boa Esperança, Cuiabá, MT 78060-900, Brazil. E-mail: valdutra@ufmt.br
Nowadays pig intensive production systems increase microbial selection pressure favoring spread of respiratory diseases. The bacteria Pasteurella multocida is associated with various respiratory diseases in pigs under farming conditions causing high economic losses. In vitro biofilm formation has been described in P. multocida which role is described in tissue colonization, enhancing resistance to host defenses and antibiotics. The aims of this study were to analyze the occurrence of P. multocida in pig pneumonia and health lung microbiota, and occurrence of tad locus genes in these isolates. Seventy isolates of P. multocida were analyzed which sixty seven were from lungs with lesion and three from healthy lungs. Serotype A occurred mainly in lung lesion (85.71%), in healthy lung, instead, only serotype D was observed. Genes tadA, tadB, tadC, tadD, tadE, tadF and tadG were present in 89.55% isolates from lesion lung. Genes tadA, tadB and tadC were present in all isolates from healthy lungs but, tadD, tadE tadF and tadG were present in 0%, 33.3%, 33,3% and 66.6%, respectively. This work associated tadD, tadE and tadF gene positive isolates of P. multocida to lung pneumonic lesions. |
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